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Image Search Results
Journal: Molecular medicine reports
Article Title: Preparation of a monoclonal antibody against the carcinoembryonic antigen, glypican‑3.
doi: 10.3892/mmr.2019.10019
Figure Lengend Snippet: Figure 3. Expression, purification and identification of recombinant GPC3 protein. (A) Induction expression of recombinant GPC3 protein by SDS‑PAGE analysis. Lane M, protein molecular weight marker; Lane 1, non‑induction of GPC3; lane 2, induction of GPC3. (B) SDS‑PAGE analysis of purification of recombinant GPC3 protein. M, standard protein molecular weight; lane 1, purified protein. (C) Confirmation of the recombinant protein by western blot analysis. The primary antibody was rabbit anti‑His‑tag monoclonal antibody. M, standard protein molecular weight; lane 1, purified protein. GPC3, glypican‑3.
Article Snippet: The recombinant plasmid was transfected into E. coli BL21 (DE3, Novagen; Merck KGaA) with the
Techniques: Expressing, Purification, Recombinant, Molecular Weight, Marker, Western Blot